LOCUS       sequence-80216-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-CprB
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             182..205
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc acagcagcgg
      181 cctggaagtt ctgttccagg ggccccatat gattgcactg agctataaag cctttctgaa
      241 cccgtatatc atcgaagttg agaaacgtct gtatgaatgc attcagagcg atagcgaaac
      301 cattaacaaa gcagcacatc acattctgag cagcggtggt aaacgtgttc gtccgatgtt
      361 tgttctgctg agcggttttc tgaatgatac ccagaaagat gatctgattc gtaccgcagt
      421 tagcctggaa ctggttcaca tggcaagcct ggttcatgat gattatatcg ataacagcga
      481 tatgcgtcgt ggtaatacca gcgttcatat tgcctttgat aaagataccg caattcgcac
      541 cggtcatttt ctgctggcac gtgcactgca gaatattgca accattaata acagcaaatt
      601 ccaccagatc tttagcaaaa ccattctgga agtgtgcttt ggtgagtttg atcagatggc
      661 agatcgtttt aactatccgg ttagctttac cgcatatctg cgtcgtatta atcgtaaaac
      721 cgccattctg attgaagcaa gctgtcatct gggtgcactg agcagccagc tggatgaaca
      781 gagcacctat catattaaac agtttggtca ttgcatcggc atgagctatc agattattga
      841 tgatatcctg
//
