LOCUS       sequence-80222-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-DhaR
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             158..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..208
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag
      181 cggcctggaa gttctgttcc aggggcccca tatggcacgt acaccggttc gtcagcatct
      241 ggttgaaaaa ggcacccagg tttttctgga acgtggttat agcggtagcg cagttcagga
      301 tattaccgca gcagcagaag ttccgaaagg tagcttttat aaccactttg aaagcaaaga
      361 agcctttggc ggtcaggttc tgcaagaatt tttcagcgat ctgcagcgta ccattagcag
      421 caccctggaa gatgcaagcg ttccgcctgt tcgtcgtctg cagaactatt ttgcagcaat
      481 tattgaaacc ctggatggtc agggttgtct gattggtaat tttagcgttg aactgagtcc
      541 gctgagtgat gttgttcgta ccgaactgct gcgtattttt gaacagtggg ttaaaccgtt
      601 ccagaaatgc attattgaag gtcaagaaac cggtagcatt cgtcgtgatc tggcaccgga
      661 tctgctggca gattttctga ttgcaagctg gcagggtgca attctgcgta tgaaaattga
      721 acgtaatcgt gaaccgctgg atcagtttct gaccgttgtt tttgaagcac tgctgccgag
      781 caccgaaaac attagcaatg aaagct
//
