LOCUS       sequence-80236-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-BarA
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             158..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..208
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag
      181 cggcctggaa gttctgttcc aggggcccca tatgcagcag cgtagcgtta ttacctgtcg
      241 tcgtctggtt accgcagcag cagaactgtt tgatcgtaaa ggttatgtta atgcaaccct
      301 gggtgaaatc accggtgcag cacgtgttac caaaggtgca ctgtattttc attttgccag
      361 caaaggtgaa ctggcacatg cagttgaaga acatggttgt accctgctgc gtccggttgg
      421 tcgtccggca cgtcgtcgtc agcagctgac cgcagcagat ccggatcgtc tgcatccgct
      481 ggcaggtccg ggtgcagccc gtggtagtcg tgcaccgcgt aattttcgtc tgagccgtga
      541 actgcgtggt ggtgaagcag ttggtggtgg ttttaccgaa gcatggatga ccaccgcatg
      601 gtcactgctg gatcgtgccg gtgcagcggg tgaactgcgt aatggtgcag atcgtgcagg
      661 caccgcagat gcagcacgtc cgcgtcgttg gcctgcaagc aattgtagcc tgcctccgac
      721 ctgtcgtacc cgtaatagcc ctccgggtag cctgccgtgt ggcaccagtc tgtgtccggg
      781 tctggttggt ccgagcggtc gtaccgatta tcgtaccaca ccgcgttaa
//
