LOCUS       sequence-80240-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A codon-optimized Cas9 nuclease under the control of the Drosophila 
            hsp70 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings 
            AM, O'Connor-Giles KM
  TITLE     Highly specific and efficient CRISPR/Cas9-catalyzed 
            homology-directed repair in Drosophila.
  JOURNAL   Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. 
            Epub 2014 Jan 29.
  PUBMED    24478335
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.113.160713"; journalName: "Genetics"; date: 
            "2014-04"; volume: "196"; issue: "4"; pages: "961-71"
COMMENT     Sequence Label: pBS-Hsp70-Cas9
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    76..948
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgcggat cccccgggct
       61 gcaggaattc ccgttagttt atttcgcaga acaagctggc gcgacacatg cgattgcaca
      121 cgggtcaaag gccgtactcc tgcaaaatgt gcagcaagag ctttgtgcag tccaacgacc
      181 ttaagatcca tatgcggcgc cacacgggcg agcgaccata tcagtgtgga gtgtgcggcg
      241 agagctttgt gtgcggttcg cacctgaata tccatcgtaa ccgaaaggga catctgattg
      301 ctgtgattcc gggcaatgaa gtagaggcaa attttgctgc tgatccttat gtaaatgcta
      361 gggtcaacca gcgccgttcg gaggatatag agcgtatgcg cctgcaacgc attcccgaaa
      421 accaattgca acagagactg gaaaatctcc ccaaaccgga tgtcccggct atgtgctaca
      481 aatgcggtgt ctgcgaacag aaattcaaaa gtggagctct cctgaccgtc catcgcaata
      541 aaatgagcca ttatgagatc gaaagggtct acgaaaatcc gttcggaaaa aatcagaaaa
      601 tcatcaaagc cgaatataat taaaatgtat tactagctaa agaaatcatc actaatatag
      661 aatgtagaat gaacccatgt atattagata ctaattgtat cgtaagactt tcaaaagtct
      721 acaagacatt aaatgacaag ttgactttaa atttcaaata aataatttat tttttctata
      781 agcaataaca tttttgctaa attaagactt ggtaattagg taatactatt gttgttctat
      841 ggaatattcg atcgaaacat tcttatcagt ctcaaaaact taaaacaaac ttataatata
      901 acccatatgt tataacccat tgatgaacaa aaattagact ctttggcctt agtcgacctc
      961 gatccgggca acgttgttgc cattgctgca ggcgca
//
