LOCUS       sequence-80241-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-CampR
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             116..138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             157..174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             184..207
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
       61 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      121 ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc
      181 ggcctggaag ttctgttcca ggggccccat atgcctcgtc agagccgtgc acgtgcaagc
      241 gcaccgaaaa aaccgaccaa acaagaacgt ctgatggcag cagcagttcg tctgtttagc
      301 cgtcagggtt atgcaggcac cagcgttcgt gatctgggtg aagcactggg tattcagcct
      361 ggtagcgttt atgcacatat cgatagcaaa cataccatgc tggttagcct ggttgaaagc
      421 ggtattgatc agtatctgga tgcagttgca gaactgcctg gcaccccgac cgaacagctg
      481 cgtcattttg ttgaagcaca tgttcgtgtt gttgccgaag atgttaatcg tgcacgtgtt
      541 gtttatcatc agtggcgtca tattcagcct ccggaacgta cccgtattgt tgcaaaacgt
      601 tatgcctatg aacatcgtct gcgcgatatt attgatgccg gtgttgcagc cggtgaattt
      661 gcaccggatc tggatcgtcc gaccgcagtt cgtgcagttc tgggtatggt taattggtgt
      721 ccggaatggc tgcctgcaga tggtagcgaa ccggctgaag cagttgcagc gggtgttgca
      781 gaaattgttc tggcaagcgt tcgtgttggt catggtgca
//
