LOCUS       sequence-80242-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-BM1P1
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..76
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg gcctggaagt
       61 tctgttccag gggccccata tgaatcagaa acagctggat attctgggtc atacccatca
      121 gctgtttatg gaaaaaggtt atgttgatac cagcgtgcag gatattctgg aacgtagcgg
      181 tattagcaaa ggcaccttct ataaatactt tagcagcaaa agcgagctgc tgattagcct
      241 gctgagcagc ctgcaagaaa gcctgattct gcagcgtgaa aaagtggtta ttgaatatcg
      301 cggtgataac cagaaaggtt ttcaagagca gatgatcttc atgatgaact ttgccgagca
      361 gaacaaaatt ccggaaatga ttgaaagcac cctggttctg aatgaagtga aaacctttca
      421 ctacatcgag gaactgaaag cagcaacctt taactggatc tatgaacgtt ttcagcagat
      481 ctttccgagc aaaaccagcc cgtacctgtt tgatggtgca gttctgctgg aaggtatgct
      541 gaataacatg tttaaaatga acaaagccct gaattatccg ctgccgattg agcagattgt
      601 tcgttattgt atgaaacgca ccgaagaaat cattattgaa gtggcagaac agaaagaaca
      661 gctgtttagc ccgagcgttt atagcgcagt g
//
