LOCUS       sequence-80244-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-BM3R1
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..74
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             684..701
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc ctggaagttc
       61 tgttccaggg gccccatatg gaaagcaccc cgaccaaaca gaaagcaatt tttagcgcaa
      121 gcctgctgct gtttgcagaa cgtggttttg atgcaaccac catgccgatg attgcagaaa
      181 atgcaaaagt tggtgcaggc accatttatc gctatttcaa aaacaaagaa agcctggtga
      241 acgaactgtt tcagcagcat gttaatgaat ttctgcagtg tattgaaagc ggtctggcaa
      301 atgaacgtga tggttatcgt gatggctttc atcacatttt tgaaggtatg gtgaccttta
      361 ccaaaaatca tccgcgtgca ctgggtttta tcaaaaccca tagccagggc acctttctga
      421 ccgaagaaag ccgtctggca tatcagaaac tggttgaatt tgtgtgcacc ttttttcgtg
      481 aaggtcagaa acagggtgtg attcgtaatc tgccggaaaa tgcactgatt gcaattctgt
      541 ttggcagctt tatggaagtg tatgaaatga tcgagaacga ttatctgagc ctgaccgatg
      601 aactgctgac cggtgttgaa gaaagcctgt gggcagcact gagccgtcag agctaaggat
      661 ccaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      721 aagcccgaaa ggaa
//
