LOCUS       sequence-80248-e        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRipTAL plasmid compatible with Golden Gate TALEN cloning system. 
            For cloning of megaTALs or other TAL-fusions and expression in 
            mammalian cells. Meganuclease/fusion is cloned between Xba-Sal 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..44
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        26..44
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(91..110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(92..110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(120..137)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(120..136)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(129..151)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(709..729)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(733..759)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(766..819)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagg
       61 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
      121 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      181 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      241 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcccgt
      301 ctccaggggg gcaccggtaa gcttccttcg cagcctatac agggcttgga ttgctggccg
      361 ctctccgtac tgggccactg tggcgatctg ggaggcggac agccgcagcg gggcggcagt
      421 aagtgcggta gccacaggca gcagggcttg gagcgccaga tctccgctcc gctgccgggc
      481 gatatccact atgtgagcgc gagtcagttc tggcagggcg gcggcgagct cggggtagtt
      541 ccgagcgacc acgcgcaaac tctgtctccg ccgagaaatt ctgcagatat cagcgtgagt
      601 aaaaccatgt ccgaccaagg cctcatgatg ctgggcaacc gtgcttcgca ctttaggctt
      661 aattttttcc tgctgctgct gtgaatagcc gagggttctg aggtctacga ctttgcgttt
      721 cttcttaggt ggcgcataat ctgggacatc atatggatac ctgcaggggc ccggattctc
      781 ctccacgtca ccgcatgtta gaagacttcc tctgccctct gaattaagct tgtgccccag
      841 tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga cgtaggtctc
      901 gttgttggcc tccttgattc tttccagtct gtagtccaca tagtagacgc caggcatctt
      961 gaggttctta gcgggtttct tggatctata tgtggtcttg atgtttgcga tcagatggct
     1021 cccgccc
//
