LOCUS       sequence-80257-e        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of GFP-tagged wild-type dynamitin p50 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Quintyne NJ, Gill SR, Eckley DM, Crego CL, Compton DA, Schroer TA
  TITLE     Dynactin is required for microtubule anchoring at centrosomes.
  JOURNAL   J Cell Biol. 1999 Oct 18;147(2):321-34.
  PUBMED    10525538
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1999-10-18"; volume: "147(2)"; pages: "321-34"
COMMENT     Sequence Label: Dynamitin-GFP
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..134
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             144..173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             183..212
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             222..251
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             261..290
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             324..353
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(396..424)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttcatc gatttaaagc tatggagcaa aagctcattt
      121 ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa
      181 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
      241 aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
      301 agagcttggg cgacctcacc atggagcaaa agctcatttc tgaagaggac ttgaattcct
      361 ctagaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      421 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      481 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      541 cacttgtcac tactttcgct tatggtgttc aatgcttttc aagataccca gatcatatga
      601 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      661 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      721 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      781 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      841 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      901 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      961 attacctgtc cacacaatct gccctttcga aagatcccca cgaaaaga
//
