LOCUS       sequence-80258-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for PCR-based transplant of Saccharomyces 
            cerevisiae GAL10/GAL1 bidirectional promoter in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Horecka J, Chu AM, Davis RW
  TITLE     IpO: plasmids and methods for simplified, PCR-based DNA transplant 
            in yeast.
  JOURNAL   Yeast. 2014 May;31(5):185-93. doi: 10.1002/yea.3006. Epub 2014 Mar 
            20.
  PUBMED    24604451
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3006"; 
            journalName: "Yeast"; date: "2014-05"; volume: "31"; issue: "5"; 
            pages: "185-93"
COMMENT     Sequence Label: pJH131
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..491)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(56..79)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    374..491
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 accctgaaac cacactcgag gacgtctgta caccgcggta tagttttttc tccttgacgt
       61 taaagtatag aggtatatta acaatttttt gttgatactt ttatgacatt tgaataagaa
      121 gtaatacaaa ctgaaaatgt tgaaagtatt agttaaagtg gttatgcagc ttttccattt
      181 atatatctgt taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg
      241 ctaaaaaact aatcgcatta tcatcctatg gttgttaatt tgattcgtta atttgaaggt
      301 ttgtggggcc aggttactgc caatttttcc tcttcataac cataaaagct agtattgtag
      361 aatctttatt gttcggagca gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg
      421 gtgaagacga ggacgcacgg aggagagtct tccgtcggag ggctgtcgcc cgctcggcgg
      481 cttctaatcc gtacttcaat atagcaatga gcagttaagc gtattactga aagttccaaa
      541 gagaaggttt ttttaggcta agataatggg gctctttaca tttccacaac atataagtaa
      601 gattagatat ggatatgtat atggt
//
