LOCUS       sequence-80260-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for PCR-based transplant of Saccharomyces 
            cerevisiae GAL10/GAL1 bidirectional promoter in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Horecka J, Chu AM, Davis RW
  TITLE     IpO: plasmids and methods for simplified, PCR-based DNA transplant 
            in yeast.
  JOURNAL   Yeast. 2014 May;31(5):185-93. doi: 10.1002/yea.3006. Epub 2014 Mar 
            20.
  PUBMED    24604451
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3006"; 
            journalName: "Yeast"; date: "2014-05"; volume: "31"; issue: "5"; 
            pages: "185-93"
COMMENT     Sequence Label: pJH131
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(514..730)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(797..820)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 tagggcgaat tgggtaccag tatattctcc agtagctagg gagcccttgc atgacaattc
       61 tgctaacatc aaaaggcctc taggttcctt tgttacttct tccgccgcct gcttcaaacc
      121 gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat
      181 tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct
      241 gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc
      301 ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc
      361 taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa
      421 gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt
      481 agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt
      541 tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa caccacatat
      601 gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct gctcggagat
      661 taccgaatca aaaaaatttc aaagaaaccg gaatcaaaaa aaagaacaaa aaaaaaaaag
      721 atgaattgaa aagctttatg gaccctgaaa ccacactcga ggacgtctgt acaccgcggt
      781 atagtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact
      841 tttat
//
