LOCUS       sequence-80262-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Valentini SR, Casolari JM, Oliveira CC, Silver PA, McBride AE
  TITLE     Genetic interactions of yeast eukaryotic translation initiation 
            factor 5A (eIF5A) reveal connections to poly(A)-binding protein and 
            protein kinase C signaling.
  JOURNAL   Genetics. 2002 Feb . 160(2):393-405.
  PUBMED    11861547
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2002 Feb . 160(2):393-405."
COMMENT     Sequence Label: pPS1597
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..20
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     28..44
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..44
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        204..868
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    420..537
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     832..855
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(937..965)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga
       61 caggttatca gcaacaacac agtcatatcc attctcaatt agctctacca cagtgtgtga
      121 accaatgtat ccagcaccac ctgtaaccaa aacaatttta gaagtacttt cactttgtaa
      181 ctgagctgtc atttatattg aattttcaaa aattcttact ttttttttgg atggacgcaa
      241 agaagtttaa taatcatatt acatggcatt accaccatat acatatccat atacatatcc
      301 atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa
      361 aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac
      421 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc
      481 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac
      541 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
      601 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
      661 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
      721 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
      781 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
      841 ctatacttta acgtcaagga gaaaaaaccc cggatctatg agtaaaggag aaaaaacccc
      901 ggatctatga gtaaaggaga agaactcttc actggagttg tcccaattct tgttgaatta
      961 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     1021 tacggaaaa
//
