LOCUS       sequence-80265-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Valentini SR, Casolari JM, Oliveira CC, Silver PA, McBride AE
  TITLE     Genetic interactions of yeast eukaryotic translation initiation 
            factor 5A (eIF5A) reveal connections to poly(A)-binding protein and 
            protein kinase C signaling.
  JOURNAL   Genetics. 2002 Feb . 160(2):393-405.
  PUBMED    11861547
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2002 Feb . 160(2):393-405."
COMMENT     Sequence Label: pPS1597
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..726
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(43..71)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     666..686
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaccccggat ctatgagtaa aggagaagaa ctcttcactg gagttgtccc aattcttgtt
       61 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      121 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      181 tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcaag atacccggat
      241 catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      301 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      361 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      421 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      481 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      541 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      601 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      661 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      721 tacaaaggat ccatgtctga cgaagaacat acctttgaaa ctgctgacgc tggttcctcc
      781 gccacctacc caatgcaatg ttctgccttg agaaagaacg gtttcgttgt catcaagagt
      841 agaccatgta agattgtcga catgtccact tctaagactg gtaagcacgg tcacgctaaa
      901 gtccatttgg ttgccattga tatcttcact ggtaagaagt tggaagattt gtctccatct
      961 actcacaaca tggaagttcc agttgtcaag agaaacgaat accaattgtt ggacattgat
     1021 gacgg
//
