LOCUS       sequence-80268-e       13218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses nine genes from the H. neapolitanus carboxysome operon and
            CSOS1D; IPTG-inducible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13218)
  AUTHORS   Bonacci W, Teng PK, Afonso B, Niederholtmeyer H, Grob P, Silver PA, 
            Savage DF
  TITLE     Modularity of a carbon-fixing protein organelle.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):478-83. doi: 
            10.1073/pnas.1108557109. Epub 2011 Dec 19.
  PUBMED    22184212
REFERENCE   2  (bases 1 to 13218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1108557109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-01-10- 10"; volume: "109"; issue: "2"; pages: "478-83"
COMMENT     Sequence Label: pHnCBS1D
FEATURES             Location/Qualifiers
     source          1..13218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(21..609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(101..120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1564..1650
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1742..1769
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1791..1810
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(1825..1830,1831..1848,1849..1855)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1863..1879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             10370..11029
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10436..10455)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        11094..11171
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             11172..12254
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(11191..11210)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctagtcgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
       61 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtc tagttcagcc
      661 agctcgtcgt gatgtcgaaa cccaagccac cctcagaggt gaaggccgct tcgagcacat
      721 cgggaagcgt gtcgacgacg gtgctcggtg cctcgggtaa gagccagata gatgcggtga
      781 cgttcaccgt cgcgatcgtg gcgggaacca cggtcactgc gtccgttaca actcgcacgt
      841 cgtcggccag cacgactgac tcgacggctt cgattagccc gggagaggca aggccatcag
      901 gctcggtaga cagaatgctg attaacacct cgccgggagc agggctgctc acagccgcgt
      961 ccttcacccg tgggtcagcc gtcagcgctt gatagcggta ccaggccgca ccgcctgcgg
     1021 tcgagctgcc cttgatccgc tcgatggtgc gatcgcgaag ctcgccatcc ggctcattcg
     1081 gctgccgcac cacgccgtag aacgcggcaa ggttgtcgag gtcggggccg tttgagtagc
     1141 gaagcagtgt cgcaagaagt gcgtcgttga tccgctgacg caggatcagc tcgcgggctg
     1201 cgcagacctc cagcagcttg atgaccgggt cggattcgag gatggcggtg tagctggcgt
     1261 cacgcgatcg caggtcgtcg atcaagtcct gcaggatcag ttcaaagtcc agcgcttcga
     1321 tgatggtggg cgcgggaatc gtagcaaagt caagaacggt catgagacga ctaagccctc
     1381 cagcgtgata cgcctgccct cggggatgta gtagccgatc aggttcagct cgacttgacc
     1441 agctgcgctg gctgagacga tgcgaacctt ctccagcttc agccgtggct cccagcgatc
     1501 gagcgcttca gctgtggccg ccaccaggtc aacgatgagg gactggttga tcggtctagt
     1561 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1621 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     1681 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     1741 cagaaggcca tcctgacgga tggccttttt gcgtttctac tctagtttcg aattgtgagc
     1801 gctcacaatt tcgaaacccc aggcttgaca ctttatgctt ccggctcgta taatgtgtgg
     1861 aattgtgagc ggataacaat ttcagctagg tgcgcgaatc ccccatcctt tcaggaggaa
     1921 ctcatggcag ttaaaaagta tagtgctggt gtaaaagaat accggcagac ctattggatg
     1981 ccggaataca caccgttgga ttccgacatc cttgcatgct tcaaaatcac cccacaaccg
     2041 ggtgttgatc gcgaagaagc cgcagccgcg gttgcagcag aatcttcaac cggcacatgg
     2101 accaccgtgt ggaccgattt gctgaccgac atggactact acaaaggccg tgcctatcgc
     2161 attgaagacg tacccggtga cgatgcggca ttctatgcct ttatcgccta cccaatcgac
     2221 ctgttcgaag aagggtcagt tgttaacgtg tttacctcac tggttggtaa cgtgttcggc
     2281 ttcaaagcgg tacgcggcct gcgtctggaa gatgttcgct tcccactcgc ctacgttaaa
     2341 acctgtggcg gcccaccgca cggtattcaa gtcgaacgcg acaagatgaa caaatatggt
     2401 cgcccactgt tgggttgcac catcaagcca aaacttggtt tgtctgcgaa aaactacggc
     2461 cgtgccgtat acgagtgcct ccgtggcggc ctcgacttca ctaaagatga tgaaaacatc
     2521 aattctcagc cgttcatgcg ctggcgcgat cgcttcttgt tcgtacaaga cgcgaccgaa
     2581 actgctgaag cccaaaccgg cgaacgcaaa ggccattacc tcaacgtaac ggcgccaact
     2641 cctgaagaaa tgtacaagcg cgcagaattc gccaaagaaa ttggcgcgcc aatcattatg
     2701 cacgactaca tcaccggtgg cttcacggcc aacactggct tggccaagtg gtgtcaagac
     2761 aacggcgtac tgctgcacat ccaccgtgcg atgcatgcgg ttatcgaccg taacccgaac
     2821 cacggtattc acttccgtgt tctgaccaag attctgcgtt tgtcgggtgg cgatcacctg
     2881 cacaccggta ccgttgtcgg caaactggaa ggcgaccgtg cctctactct gggctggatt
     2941 gatttgctcc gcgaatcgtt tatccctgaa gatcgctcgc gcggtatctt cttcgatcaa
     3001 gactggggtt caatgccagg cgtattcgct gtggcctctg gtggtattca cgtatggcac
     3061 atgcctgcgc tggttaacat ctttggtgac gactctgtcc tccaattcgg tggcggtacg
     3121 ctgggtcatc catggggcaa cgctgccggt gctgctgcca accgtgttgc tctggaagcc
     3181 tgcgtagaag cgcgtaacca aggccgcgat atcgaaaaag aaggcaaaga aattctgact
     3241 gctgctgcac agcacagccc agaactgaag attgccatgg aaacttggaa agagatcaaa
     3301 ttcgaatttg acactgtcga caaactcgac actcaaaatc gttgatccct cgtaccacac
     3361 aacatactaa ggtgagtaac catggctgaa atgcaggatt acaagcaaag cctcaaatat
     3421 gagactttct cttatcttcc acccatgaac gcggaacgca tccgcgctca aatcaagtac
     3481 gcaattgctc aaggctggag ccccggcatt gagcacgtag aagtgaaaaa ctccatgaac
     3541 caatattggt acatgtggaa acttcccttc ttcggcgaac aaaatgtcga caacgtgttg
     3601 gctgaaattg aagcgtgtcg tagtgcgtat ccaacacacc aggtcaaact ggtggcttat
     3661 gacaactatg cgcaaagctt aggtctggcc ttcgtggtct accgcggcaa ctaagtcaag
     3721 tctgtcattg cgctgtgcgt ttctctacgc acagcactta ttcaagatgg ggtcaaacat
     3781 gccttcacag tcaggaatga atcctgccga cctgagcgga ctctctggca aggaactggc
     3841 acgcgcacgc cgcgctgcac tatccaagca agggaaagca gctgtttcta ataaaacggc
     3901 tagcgtaaac cgtagcacta aacaggcggc atcttcgatc aatacaaatc aggtgcgctc
     3961 ttctgtaaat gaagtgccca ctgattacca aatggcggat caattgtgct ctacgattga
     4021 tcatgctgac tttggtaccg aaagcaatcg cgttagagat ctctgccgcc aacgcagaga
     4081 ggcactatca actatcggta aaaaagcggc taaaaccacc ggcaagccgt cgggtcgcgt
     4141 tcgaccacag caatcagtgg ttcacaacga cgcaatgatc gaaaatgccg gtgatactaa
     4201 ccaatcatcg tccacttcat taaataatga actttccgaa atctgctcca tagcagacga
     4261 catgccggag cgttttggtt cacaagccaa aaccgtccgt gatatctgcc gtgcacgccg
     4321 tcaagcgctc tctgagcgtg gaactcgcgc cgtgccgcca aagccgcaat ctcaaggtgg
     4381 tccaggacgc aatggctatc aaattgatgg atacctagat accgcacttc atggccgcga
     4441 tgccgccaag cgccaccgtg aaatgctctg tcaatacggc cgcggcacag caccttcctg
     4501 caagccaaca ggccgtgtca aaaattctgt acagtcgggc aacgcagcgc caaaaaaggt
     4561 tgaaaccggt cacaccctat ccggcggatc tgttacgggc acgcaagtgg atcgtaaatc
     4621 tcatgtgact ggaaacgagc cgggcacttg ccgagcagtc acgggcaccg agtacgtagg
     4681 tactgagcaa ttcacctctt tttgcaatac cagccccaag ccaaatgcga cgaaggtcaa
     4741 tgtgaccaca acggctcgtg gtcgccctgt tagcggtacg gaagtttcac ggaccgagaa
     4801 ggtaactggc aacgaatccg gtgtctgccg taacgttacc ggcaccgaat acatgagtaa
     4861 tgaagctcac ttttctctat gtggaacagc cgcaaagcct tcacaagcgg ataaagtcat
     4921 gttcggcgcc acagcacgaa cgcatcaagt ggtcagtggc agtgatgaat tcaggccctc
     4981 ttctgttacg ggtaacgaat cgggtgcaaa acgcacaatt accggctcgc agtacgcaga
     5041 cgaaggtctt gcgcgactca cgatcaacgg agcacctgca aaagtagcca gaacccacac
     5101 ctttgcgggc tctgacgtta ccggcacgga aatcggtcgc tctactcgcg taactggtga
     5161 tgaaagcggt tcgtgtcgtt caatctcagg caccgagtat ctcagtaacg agcaattcca
     5221 atccttttgt gacacaaaac ctcaacgcag cccgttcaag gttggccaag atcgcacgaa
     5281 caagggtcag tctgtgactg gtaacttggt tgatcgttcc gaactggtta caggtaacga
     5341 accaggttca tgctctcggg ttacaggctc tcagtatggc caaagcaaaa tctgcggtgg
     5401 tggcgtggga aaagtgcgct caatgcgcac ccttcgcggc acctcagtat ctggccaaca
     5461 gctagatcat gccccaaaga tgtccggtga cgagcgcggc gggtgcatgc ccgtcaccgg
     5521 taatgagtac tacggtcgtg aacatttcga accgttttgt acgagcaccc cagagcccga
     5581 agctcaatca actgaacaat cattgacctg tgaaggacaa attattagcg gcacttcagt
     5641 tgacgccagt gatttggtca caggaaatga aatcggtgaa cagcaactca tcagcggtga
     5701 cgcctatgtt ggcgcgcagc agacaggttg ccttcccact agtccacgct tcaaccaaac
     5761 tggcaatgtt cagtcaatgg gttttaagaa caccaatcag ccagaacaaa actttgcacc
     5821 aggtgaagta atgcctactg actttagtat tcaaacccca gctcgctcgg ctcagaatcg
     5881 cattacaggt aacgacattg cgccctcagg tcgcattaca ggccctggta tgctggcaac
     5941 cggcttgatt acaggaaccc ccgaattcag gcacgctgcg cgcgagttgg ttggttctcc
     6001 acaacccatg gcaatggcca tggccaaccg taataaggct gctcaagcac ctgttgtgca
     6061 gccagaagtg gttgcaactc aggaaaagcc tgagttggta tgtgcaccaa gaagcgatca
     6121 aatggatcgt gtgagtggcg aaggcaaaga acgttgccac atcactggcg atgactggtc
     6181 agttaacaag cacatcaccg gtacagccgg tcaatgggcg agtggtcgca acccttccat
     6241 gcgcggtaat gcgcgtgtgg tcgaaaccag cgcgtttgcc aatcgcaatg tgccaaaacc
     6301 tgaaaagccg ggctccaaga tcacgggcag tagtggtaat gacacccaag gtagtctgat
     6361 cacttactcc ggcggcgcgc gcggttgatt aagtaaagtg taacgatcat gaacacccgt
     6421 aacacacgaa gcaagcaacg cgcaccgtgt ggtgttagct catcagtcaa acctcggctt
     6481 gacttgattg agcaagcacc aaaccctgtc tatgaccgcc atcctgcttg tataacgttg
     6541 cctgagcgta cctgccggca cccgttaact gaccttgaag ccaacgaaca actgggtcgt
     6601 tgcgaggata gcgtcaagaa ccggtttgat cgcgttatcc ctttcttgca agttgttgct
     6661 ggcattcctc ttggtctgga ttatgttacc cgtgttcaag aattagccca gtcgtcgctt
     6721 ggacatacgc tgcccgaaga attactcaaa gataattgga tcagtggaca caatttaaaa
     6781 ggcatttttg gctacgcgac tgctaaagca ctaacagcag ccacggaaca attcagtcgt
     6841 aaaataatgt ctgagaagga cgattccgca tcggccattg gcttcttcct ggattgcggg
     6901 ttccacgcag tggacataag cccttgtgcc gatggtcgtc tcaaagggtt actgccttac
     6961 atattgcgtt tgccccttac ggcattcacc tatcgtaaag cctacgcagg ctcgatgttc
     7021 gatattgaag atgatctggc acagtgggag aaaaatgaac tccgccgtta tcgtgaaggt
     7081 gttccaaata cagcggatca gccaacacga tacctgaaaa ttgctgtgta tcatttcagc
     7141 acctctgacc cgacacactc tggctgcgcg gcacacggca gtaatgatcg tgcagcactg
     7201 gaagcggctt taacccagct gatgaaattc agagaagcgg ttgaaaatgc ccattgctgc
     7261 ggcgcaagta tcgatatttt actgattggc gttgatacgg atacggatgc cattcgcgtt
     7321 catattccgg atagcaaagg ttttttgaat ccgtatcgtt atgttgacaa cacagtaact
     7381 tatgcgcaaa cactacatct ggcgccggat gaggctcgtg tgattattca cgaagcaatt
     7441 ctcaacgcaa accgcagcga tggttgggct aaaggaaatg gagtagccag cgaggggatg
     7501 cgtcgtttta ttggtcagct tttgatcaac aacctctcgc aaatcgatta cgtagtaaat
     7561 cgtcatggtg gtcgctatcc acccaatgat attggtcatg ctgagcgata tatcagtgtt
     7621 ggtgatggtt ttgatgaagt tcaaatccgg aatttagcct actacgcgca tttggatacg
     7681 gttgaagaaa atgcgattga tgtggatgtg ggaatcacaa ttttcaccaa acttaatttg
     7741 agtcgaggtt taccgattcc gattgccatc cactatcgct atgaccccaa tgttccaggc
     7801 tccagagaaa gaaccgtggt caaagcaaga cggatataca acgccattaa agagcggttc
     7861 tcatccttgg atgagcagaa tctattgcag tttcgtttga gcgttcaggc gcaggatatc
     7921 ggaagcccga ttgaagaggt tgcatccgca tgaaaatcat gcaagttgag aaaacgttgg
     7981 tttcaacaaa ccgtattgct gatatgggtc acaaaccact attagtggta tgggagaaac
     8041 cgggcgcgcc caggcaggtc gccgtggatg cgattggctg cataccgggc gattgggttt
     8101 tgtgcgttgg gtcatcggca gcacgagagg ctgcaggaag caagtcttat ccctctgatt
     8161 tgacgattat cgggattatt gatcagtgga atggtgagta atggaagtaa tgcgcgttcg
     8221 ttccgaccta atcgcaacac gcaggattcc cggtcttaaa aatatctctt tgcgtgtcat
     8281 ggaggatgct acgggtaagg tcagtgtcgc ttgcgatccc attggcgttc ccgagggatg
     8341 ttgggtcttt acgattagcg gctctgccgc tcggtttggc gtgggtgatt ttgagattct
     8401 cacggatttg acgattggtg gcatcatcga tcactgggta acttgaccat cgctagatga
     8461 gttgattttg aatgagtctt tattgaggag agaagaaatg gcagcagtaa caggtattgc
     8521 actgggtatg attgaaacac gtggtctggt tccagcgatt gaagctgccg atgccatgac
     8581 caaggccgcc gaagtacgtt tggttggccg tcaatttgtt ggtggtggtt acgtgaccgt
     8641 tttggtccgt ggtgaaaccg gtgccgtcaa cgcagcagtt cgtgcgggcg ctgatgcctg
     8701 cgaacgagtc ggcgatggtc tggtcgcggc gcatatcatt gcccgtgtcc attccgaagt
     8761 cgaaaacatc ctgccgaaag cccctgaagc ttaaggattg ggaaagacga accggcgcag
     8821 gcttgttccg gttctttgca taaagtgaca gcttaggagt ttatttaaat ggctgatgta
     8881 actggtattg ctctgggtat gatcgaaaca cgtggcttag ttcctgcgat tgaagcagcg
     8941 gacgccatga ctaaagcggc tgaagtgcgt ttggtcggtc gtcaatttgt tggtggcggt
     9001 tacgtcaccg tattggttcg gggcgaaaca ggcgctgtta acgccgctgt tcgtgctggc
     9061 gccgatgctt gcgaacgtgt tggtgatggc ttggttgctg cgcacatcat tgcgcgtgtc
     9121 cactcagaag tagaaaacat tctgcctaag gcgccacaag cctaagtcag atattcctaa
     9181 gacggctcac ttcggcgaca cccgtcgccg aaaccgtcta accaaatttc tgggctcgtt
     9241 ctgaacctgc tcaacactag ttttagagga tctgttatgg caacgactca cggtattgcc
     9301 ctgggcatga ttgaaacacg aggattggtt cctgccattg aagccgcaga tgccatgacc
     9361 aaagcggcgg aagtccgtct ggtcggacga tcatttgttg gcggcggtta cgtgaccgta
     9421 atggttcgtg gtgagacagg tgcagtaaat gctgccgttc gtgcgggtgc tgacgcctgt
     9481 gaacgtgttg gcgatggcct ggttgctgcg cacatcattg cgcgcgttca ttctgaagtt
     9541 gagatcatcc tacccgagac gcccgaagac tcagattccg cgtggtgtat cgcaaatctg
     9601 aatagctaat gtctagtagg gaagatgcgc atgaacaaca ttgatttgcg cgtctattcg
     9661 tttatcgatt cgcttcaacc gcagcttgca tcctatcttg cgacatcatc gcaaggcttt
     9721 cttcccgttc cgggtgatgc ctgcttgtgg attgaagtcg cgccgggcat ggccgtccat
     9781 cgcctcagtg atattgcgct aaaggccacg aacgttcgtc tcggcgaaca ggtagtcgag
     9841 cgtgcttttg gctcgatgga aattcattac cgaaaccaaa gcgacgttct cgcatccggt
     9901 gaggccgttt taagagaaat caatcacgcg caagaagatc gtctgccttg tcgcatcgca
     9961 tggaaagaga tcattcgagc gattaccccc gatcatgcca ccttgatcaa tcgccagttg
    10021 cgtaaaggct ctatgctgtt gccgggcaaa agcatgttca tccttgaaac agaaccggca
    10081 ggttatattg ttcaggctgc caacgaggcc gagaaagcag ctcatgtgac tctgatcgat
    10141 gtacgtgctt ttggtaactt tggtcgcctg accatgatgg gcagcgaagc ggaaaccgaa
    10201 gaagccatgc gggcggctga ggccacaatc gcaagcatca acgcgcgtgc gcgtcgcgct
    10261 gaagggttct aagcggccgc gaggttccaa ctttcaccat aatgaaataa gatcactacc
    10321 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
    10381 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
    10441 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
    10501 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
    10561 ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtgat
    10621 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
    10681 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
    10741 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
    10801 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
    10861 caacttcttc gcccccgttt tcactatggg caaatattat acgcaaggcg acaaggtgct
    10921 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat
    10981 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
    11041 cagttattgg tgcccttgaa ttcctactag tcgaagcggc atgcatttac gttgacacca
    11101 tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
    11161 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
    11221 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
    11281 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
    11341 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
    11401 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
    11461 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
    11521 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
    11581 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
    11641 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
    11701 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
    11761 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
    11821 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
    11881 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
    11941 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
    12001 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
    12061 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
    12121 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
    12181 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    12241 aaagcgggca gtgatcccac agccgccagt tccgctggcg gcattttaac tttctttaat
    12301 gaatctagtg acaagccggg gcagacgtga gccgtagtcc cgtcgccaga cgcgggtgcc
    12361 cacgggcgtc gtcaggatgt ccgtaattga ctgccggagg tggtcaatgc ccttcagctc
    12421 cttgccactg tcacggctca tgcctcgggt cattagtcgc ccgctccggt atcttcactg
    12481 gcttcgatga ttgccgcccc gcagctgcag aggtcaccga tccgagcagt cggcctctgg
    12541 ttggtaaaga ccgtgcgact gccggtgatg atcgtgttca agccatgcag ggggcaggcg
    12601 tgaaggtcat cctttcgggc cacggggcgg ctgttgacga aggtgtcgtc gctcccggtg
    12661 atgatgatcc cgccgtgatc ggtcacgtcg tttagtcgag cgatgcctgg cgtcgtagtc
    12721 acgggtttag gtcaatacga cttgcggtca ctgtaacgtt gccctcggcg gtcacgttaa
    12781 cgtcgccttg ggcttcgact tgcgcctcct gcacaaggat cacaatccgt ccttgggctg
    12841 cggtgaggtc gatcttgtac tcatgcgctt cgcggtcgta ctggatgatt gagtcatcct
    12901 cgaactgcgt cttttggatc gtttctttgt cctcgatctg ggggtagtca gtcgagaacg
    12961 cgccgggcat cgcgaagccc tgactgatct cgccggaggg ggccatcacg acgacggcct
    13021 caccgacctc gggcgcccac cagaaccgat ccttgcccgc tcgctgcgtg agccacggaa
    13081 tccagtcagt gaggagcagc gcctcgccgc tctcctcgtc ttcctcgatc gcgacacgga
    13141 tcagcccctt gggatagtca gcctcggcta ccctgcctac gcggagcaag ttgccgtgac
    13201 gccgactgtc tcgagtat
//
