LOCUS       sequence-80281-e        9176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.hygro
FEATURES             Location/Qualifiers
     source          1..9176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    72..189
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(535..555)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        540..870
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      721..856
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             919..1944
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             919..1944
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    2074..2195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2303..2536
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2608..2729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2769..2904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2924..2943)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2925..2943)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2953..2970)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2953..2969)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2962..2984)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3111..3566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3198..3217)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3408..3429
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3592..3696
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3697..3765,3766..4557)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3915..3934)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5147..5166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5400..5417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5570..5586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5570..5586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5605..5625
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5607..5625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5653..5879
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5880..6060
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6107..6232
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6725..6958
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7143..7187
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    7464..7588
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7613..7618,7619..7636,7637..7643)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7697..8356
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7763..7782)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8698..9003
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8882..8901
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9044..9168)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggccgctcta gagggcccaa gcttacgcgt gaattcgtcg actcgagaca aatggcagta
       61 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      121 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      181 caaaattttc gggtttatta cagggacagc agagatccag tttggccgcg gctcgagggg
      241 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
      301 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
      361 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
      421 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
      481 agccgcacgt ctcactagtg gatcccccgg gctgcaggaa ttcgataatt ctgtggaatg
      541 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
      601 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      661 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      721 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      781 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
      841 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
      901 ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
      961 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     1021 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     1081 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     1141 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     1201 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     1261 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     1321 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     1381 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     1441 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     1501 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     1561 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     1621 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     1681 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     1741 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     1801 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     1861 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     1921 actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc
     1981 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     2041 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     2101 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2161 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctcg
     2221 agggggggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2281 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     2341 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2401 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2461 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2521 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     2581 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     2641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2701 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     2761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2821 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2881 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     2941 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3001 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3061 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     3121 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3181 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3241 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3301 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3361 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3421 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     3481 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3541 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     3601 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3661 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3721 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3781 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3841 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3901 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3961 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4021 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4081 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4141 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4201 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4261 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4321 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4381 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4441 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4501 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4561 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4621 aggatctagg tgaagatcct ttttgataat ctcatgagga agcggaagag cgctgcgcgt
     4681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4741 agagctacca actctttttc cgaaaggtaa ctggcttcag cagagcgcag ataccaaata
     4801 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4861 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4981 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5281 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5341 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5401 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5461 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5521 agcgcaacgc aattaatgtg agttagctca ctcggataac aatttcacac aggaaacagc
     5581 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     5641 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     5701 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     5761 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     5821 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     5881 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5941 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6001 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6061 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6121 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     6181 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     6241 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     6301 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     6361 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6421 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     6481 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     6541 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     6601 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     6661 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     6721 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     6781 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     6841 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     6901 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     6961 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     7021 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     7081 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     7141 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     7201 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     7261 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     7321 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     7381 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     7441 ggtatcgatc ctcgagccca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     7501 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     7561 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     7621 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     7681 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     7741 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     7801 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     7861 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     7921 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     7981 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     8041 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     8101 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     8161 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     8221 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     8281 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     8341 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta
     8401 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     8461 aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     8521 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     8581 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     8641 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg
     8701 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     8761 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     8821 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     8881 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     8941 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     9001 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     9061 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     9121 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atgggc
//
