LOCUS       sequence-80286-e        8897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.YFP
FEATURES             Location/Qualifiers
     source          1..8897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(114..238)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(300..323)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     704..723
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(796..815)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     regulatory      947..956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(953..955,956..958,959..1672)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(998..1019)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1259..1278)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1606..1627
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1797..1817)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     LTR             2039..2272
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2344..2465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2381..2400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2435..2454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2505..2640
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2781..3236
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2868..2887)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3078..3099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3262..3366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3367..3435,3436..4227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3585..3604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4817..4836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5070..5087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5240..5256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5240..5256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5275..5295
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5277..5295
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5323..5549
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5550..5730
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5777..5902
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6395..6628
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6813..6857
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7156..7404
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7156..7176
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7327..7346
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    7431..7555
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7580..7585,7586..7603,7604..7610)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7664..8323
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7730..7749)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             join(8665..8897,1..73)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8849..8868
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
       61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
      121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
      241 atgggcggcc gctctagagg gcccaagctt acgaggcgct tttcccaagg cagtctggag
      301 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
      361 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
      421 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
      481 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
      541 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
      601 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
      661 gcggggcggg cgcccgaagg tcctccggag gcccggtgtt ccgcattctg cacgcttcaa
      721 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
      781 ccaagctagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
      841 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc catcggctcc
      901 ctcgttgacc gaatcaccga cctctctccc cagggggatc ccggtcgcca ccatggtgag
      961 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1021 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1081 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1141 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1201 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1261 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1321 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1381 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1441 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1501 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1561 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1621 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     1681 cgactctagc atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc
     1741 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1801 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1861 cgtatggctt tcattttctc ctccttgacc ggagcttacc cgcccgcccc acgacccgca
     1921 gcgcccgacc gaaaggagcg cacgacccca tgcatcgtta agagctcggt acctttaaga
     1981 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     2041 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     2101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     2281 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     2341 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2401 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2461 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     2521 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2581 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2641 tagggacgta cccaattcgc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2701 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2761 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2821 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2881 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2941 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3001 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3061 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3121 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3181 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     3241 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3301 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3361 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3421 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3481 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3541 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3601 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3661 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3721 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3781 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3841 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3901 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3961 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4021 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4081 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4141 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4201 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4321 ctcatgagga agcggaagag cgctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4381 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaaggtaa
     4441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5161 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5221 ctcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     5281 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     5341 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     5401 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     5461 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     5521 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     5581 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5641 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5701 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     5761 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     5821 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     5881 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     5941 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     6001 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     6061 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     6121 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     6181 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     6241 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     6301 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     6361 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     6421 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     6481 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     6541 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     6601 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     6661 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     6721 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     6781 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     6841 ataaatgggc aagtttgtgg aattgggttt aacataacaa attggctgtg gtatataaaa
     6901 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     6961 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     7021 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     7081 agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg
     7141 gccgccccct tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac
     7201 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     7261 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     7321 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     7381 atcttgtgga aaggacgaaa caccggccgg tgatatcctc gagcccatca acaagtttgt
     7441 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     7501 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     7561 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     7621 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     7681 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     7741 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     7801 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     7861 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     7921 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     7981 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     8041 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     8101 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     8161 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     8221 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     8281 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     8341 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     8401 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     8461 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     8521 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     8581 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     8641 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     8701 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     8761 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     8821 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     8881 gtctccgtta tcgggga
//
