LOCUS       sequence-80288-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Forebrain principle neuron specific expression of GCaMP6f Ca sensor 
            and physically separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..111
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(115..123,124..138)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             166..222
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     435..456
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(562..583)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aggcgagtgg cccctagttc tgggggcagc gggggatccg gcgccaccat gggttctcat
       61 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      121 tacgacgatg acgataagga tctcgccacc atggtcgact catcacgtcg taagtggaat
      181 aagacaggtc acgcagtcag agctataggt cggctgagct cactcgagaa cgtctatatc
      241 aaggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
      301 gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc
      361 gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac
      421 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      481 atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg
      541 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      601 agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc
      661 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      721 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      781 atgcccgaag gctacatcca ggagcgcacc atcttcttc
//
