LOCUS       sequence-80291-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to
            Ala .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(131..154)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact
       61 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtctagata
      121 tctcgactca cttgtcatcg tcgtccttgt aatctccgcc agatcctggt gttccgccac
      181 gcgtctgcat aactgcaagg aacttgcttt cttctgcaag ggaggtcagc aaagaactca
      241 tatccccgat agccatgttg gtggtgccca tggataggga tgggaatggg agggatgccc
      301 gcggagtggt gagacgggag gagctgtggg aaaggttctg aataatactt gggctcaagg
      361 cccctgacat taggctggta tggtccccat catctatgat ggcatcaaaa tcaatctgca
      421 caccttctag gtcatgactc tcaaagctgt caactgtgct tgttacctgg ttagcacctg
      481 gggagagtaa ctcagagttt tcagtgccat tcccttggag caggcagttg gcatctgaga
      541 gggagaacga gccagctttc ctgtcttgct gactgaagcc catggtttgg tcatagaact
      601 gaccggaata attctgcacg gtagagcaga gctgctggga ctgaccttgt gcctccattt
      661 tgatgctgtt caccctgctg ctctgactca tgtcactgag ctgtccttgt tgcagagtga
      721 ggttgcccct tggcattgcc tgacgcctgt tacccccata gctggcacag ggctggtagc
      781 ccctgatagc tgccatgctt gatggctggc tgctgttgtc ctgagtccct ggtaggcagc
      841 tctctgtccc ttgatagatg ttgatatgtg aggtagcacc aacctgccct agtacc
//
