LOCUS       sequence-80310-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-AmtR
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             171..194
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc ctggaagttc
      181 tgttccaggg gccccatatg gcaggcgcag ttggtcgtcc gcgtcgtagt gcaccgcgtc
      241 gtgcaggtaa aaatccgcgt gaagaaattc tggatgcaag cgcagaactg tttacccgtc
      301 agggttttgc aaccaccagt acccatcaga ttgcagatgc agttggtatt cgtcaggcaa
      361 gcctgtatta tcattttccg agcaaaaccg aaatctttct gaccctgctg aaaagcaccg
      421 ttgaaccgag caccgttctg gcagaagatc tgagcaccct ggatgcaggt ccggaaatgc
      481 gtctgtgggc aattgttgca agcgaagttc gtctgctgct gagcaccaaa tggaatgttg
      541 gtcgtctgta tcagctgccg attgttggta gcgaagaatt tgcagaatat catagccagc
      601 gtgaagcact gaccaatgtt tttcgtgatc tggcaaccga aattgttggt gatgatccgc
      661 gtgcagaact gccgtttcat attaccatga gcgttattga aatgcgtcgc aatgatggta
      721 aaattccgag tccgctgagc gcagatagcc tgccggaaac cgcaattatg ctggcagatg
      781 caagcctggc agttctgggt gcaccgctgc ctgcagatcg tgttgaaaa
//
