LOCUS       sequence-80312-e         319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contain AAV ITRs, lacZ expressing for blue-white selection for 
            golden-gate reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Luo Y, Lin L, Bolund L, Sorensen CB
  TITLE     Efficient construction of rAAV-based gene targeting vectors by 
            Golden Gate cloning.
  JOURNAL   Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. 
            eCollection 2014 May.
  PUBMED    24806227
REFERENCE   2  (bases 1 to 319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114169";
            journalName: "Biotechniques"; date: "2014-05-1- 1"; volume: "56"; 
            issue: "5"; pages: "263-8"
COMMENT     Sequence Label: pGolden-AAV
FEATURES             Location/Qualifiers
     source          1..319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4..22
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(70..90)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(70..88)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(109..125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(109..125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(122..144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    133..149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(157..163,164..181,182..187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    202..223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggtcctccag
       61 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
      121 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      181 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      241 gcccgctttc caccggtggt ctctggcggc ggccgcagga acccctagtg atggagttgg
      301 ccactccctc tctgcgcgc
//
