LOCUS       sequence-80313-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate 
            reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Luo Y, Lin L, Bolund L, Sorensen CB
  TITLE     Efficient construction of rAAV-based gene targeting vectors by 
            Golden Gate cloning.
  JOURNAL   Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. 
            eCollection 2014 May.
  PUBMED    24806227
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114169";
            journalName: "Biotechniques"; date: "2014-05-1- 1"; volume: "56"; 
            issue: "5"; pages: "263-8"
COMMENT     Sequence Label: pGolden-Hyg
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcatctccg ggcctttcga atggatagat ccggaaagcc tgaactcacc gcgacgtctg
       61 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
      121 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
      181 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
      241 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
      301 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
      361 ttctgcagcc ggtcgcggag gccatgggtg cgatcgctgc ggccgatctt agccagacga
      421 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
      481 tatgcgcgat tgctaatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
      541 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
      601 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
      661 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
      721 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
      781 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
      841 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
//
