LOCUS       sequence-80316-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP160 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC154-dual-dCas9VP160-sgExpression
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        118..366
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     118..138
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     289..308
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        385..460
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtgag
      121 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      181 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      241 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      301 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      361 aacaccgggt cttcgagaag acctgtttta gagctagaaa tagcaagtta aaataaggct
      421 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga
      481 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa
      541 tggctctaga ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      601 acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat
      661 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      721 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca
      781 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
//
