LOCUS       sequence-80319-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate 
            reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Luo Y, Lin L, Bolund L, Sorensen CB
  TITLE     Efficient construction of rAAV-based gene targeting vectors by 
            Golden Gate cloning.
  JOURNAL   Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. 
            eCollection 2014 May.
  PUBMED    24806227
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114169";
            journalName: "Biotechniques"; date: "2014-05-1- 1"; volume: "56"; 
            issue: "5"; pages: "263-8"
COMMENT     Sequence Label: pGolden-Hyg
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..165)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        177..676
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(212..235)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     609..628
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gactcactat agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat
       61 ggatatctgc agaattcgcc cttaggtctc acgtgctaaa gggaacaaaa gctggagctc
      121 caccgcggtg gataacttcg tatagcatac attatacgaa gttatcgcgc cctaccgggt
      181 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
      241 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
      301 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
      361 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
      421 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
      481 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
      541 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
      601 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttcc cctcttcctc
      661 atctccgggc ctttcgaatg gatagatccg gaaagcctga actcaccgcg acgtctgtcg
      721 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
      781 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
      841 gctgcgccga tggtttctac aaagatcgtt atgtttatcg
//
