LOCUS       sequence-80321-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate 
            reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Luo Y, Lin L, Bolund L, Sorensen CB
  TITLE     Efficient construction of rAAV-based gene targeting vectors by 
            Golden Gate cloning.
  JOURNAL   Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. 
            eCollection 2014 May.
  PUBMED    24806227
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114169";
            journalName: "Biotechniques"; date: "2014-05-1- 1"; volume: "56"; 
            issue: "5"; pages: "263-8"
COMMENT     Sequence Label: pGolden-Neo
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..128
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    153..377
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     366..383
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(526..545)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 actcactata gggcgaattg ggccctctag atgcatgctc gagcggccgc cagtgtgatg
       61 gatatctgca gaattcgccc ttaggtctct ggacataact tcgtataatg tatgctatac
      121 gaagttatag ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg
      181 cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat
      241 agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt
      301 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
      361 gcaactagaa ggcacagtcg aggctgatca gcgagctcta gagaattgat cccctcagaa
      421 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
      481 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
      541 caacgctatg tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga
      601 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
      661 atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
      721 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
      781 tcgctcgatg cgatgtttcg cttggtggt
//
