LOCUS       sequence-80325-e         348 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contain AAV ITRs, lacZ expressing for blue-white selection for 
            golden-gate reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Luo Y, Lin L, Bolund L, Sorensen CB
  TITLE     Efficient construction of rAAV-based gene targeting vectors by 
            Golden Gate cloning.
  JOURNAL   Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. 
            eCollection 2014 May.
  PUBMED    24806227
REFERENCE   2  (bases 1 to 348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114169";
            journalName: "Biotechniques"; date: "2014-05-1- 1"; volume: "56"; 
            issue: "5"; pages: "263-8"
COMMENT     Sequence Label: pGolden-AAV
FEATURES             Location/Qualifiers
     source          1..348
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..32
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14..32
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(79..98)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(80..98)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(108..125)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(108..124)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(117..139)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggaggac ctcgaggggg
       61 ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
      121 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      181 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      241 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgccggtct cgataggcgg
      301 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgct
//
