LOCUS       sequence-80327-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of human His-DJ1 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     MJFF plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET3a-His-DJ1
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             599..616
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(673..691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      687..734
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatggcaagc aagcgcgcac tggtgattct
       61 ggccaagggc gccgaagaga tggaaaccgt gatcccggtt gatgtgatgc gccgcgccgg
      121 tattaaagtg accgtggcag gtctggccgg caaagatccg gtgcagtgca gccgcgatgt
      181 ggtgatttgt ccggatgcca gcctggaaga tgccaaaaaa gaggggccgt atgatgtggt
      241 ggttctgccg ggcggcaatc tgggtgccca gaatctgagc gaaagcgccg ccgtgaagga
      301 gatcctgaaa gagcaggaga accgcaaagg cctgattgcc gccatttgtg caggcccgac
      361 cgcactgctg gcccatgaaa ttggcttcgg tagcaaggtt accacccacc cgctggccaa
      421 agataagatg atgaacggcg gccactacac ctacagcgaa aaccgcgtgg aaaaagatgg
      481 cctgattctg accagccgcg gtccgggtac cagctttgaa ttcgccctgg caatcgttga
      541 ggccctgaac ggcaaagaag tggccgccca ggtgaaagcc ccgctggttc tgaaagacca
      601 ccatcatcac catcattaat aaggatccgg ctgctaacaa agcccgaaag gaagctgagt
      661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      721 tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc
      781 gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg
      841 ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaa
//
