LOCUS       sequence-80330-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniMos transposable element with HygroR and peel-1, R4-R3 Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1656
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(118..241)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(261..277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(261..277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(766..785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggggagagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
       61 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat
      121 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
      181 tttaatatat tgatatttat atcattttac gtttctcgtt caacttttct atacaaagtt
      241 gatagcttgg cgtaatcatg gtcatagctg tttcctggat ccggccgcag ctgttttctt
      301 caaatgaaaa cagaaaatta atactgtccg cgtttgctct ttattcggca cgaaactcga
      361 catgttgact gcactgagag taaacaatta tgacgctcaa ttcgcgccaa actatggtgg
      421 ttcgacagtc aaggttgaca cttcacaagg tcaaagtttt atgacaatcg ataaatattt
      481 acgtttgcga gacatctata tgttcgaaac gacatttcat acttgtacac ctgatagaat
      541 tcctcgagac gtacggtgcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgta
//
