LOCUS       sequence-80332-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DICE donor plasmid without neural transcription factors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attPC
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..31
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    235..284
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             complement(416..475)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(488..541)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(602..664)
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
ORIGIN
        1 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg
       61 cggccgctct agaactagtt gggcgcgccc aatttaaacg gttaattaat ctccgaactt
      121 cccaaggggc agggcttcaa cgccgccacg ctggagttcg gtgatctggt ctccgccggc
      181 gtcgtcgatc cggccaaggt gacccgctcg gctgttctca acgccgcctc ggtggcccgg
      241 atgatcctga cgacggagac cgccgtcgtc gacaagccgg ccgacgagga cgagcacggc
      301 cacggccacc accacggcca cgctcactga tccatttcac gcgacacccc cggtccgctt
      361 gggccggggg tgttttcgtt tgggtgcggg cgccgctgag cggtttttca ccggtgggac
      421 ctgggttgct ctcaacatct ccagccaatt tcaagagagc ataattagta cactgtacgt
      481 agatatcagg gccgggattc tcctccacgt caccgcatgt tagaagactt cctctgccct
      541 ctcgcgagac gtcaggcccg gggttttctt caacatctcc tgcttgcttc gtggccacgt
      601 gttaagcggg tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg
      661 cgaaaagtct tggagccacc gccaggtgtt tgcagccggg agttctcagc ctccaggagg
      721 gtcctggaga ggacagaggg aaagtggggt cagccctctg cccccaggcc tgcaggcagc
      781 aagccagctg gggacaactc aggctgagct tgcaccatcc aggccctgca ggcttcctct
      841 tcatttgaca ctgtcacaca aaagccacac tcagggtgaa atcacaca
//
