LOCUS       sequence-80334-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniMos transposable element with HygroR and peel-1, R4-R3 Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1656
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(25..348)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
ORIGIN
        1 ctcttcgcta ttacgccagc ttgagacttt tttcttggcg gcacaataaa gtttcctctt
       61 tttgttgggt cactcaacga acacaataca aatatttgag cgctcgcatt tttctcaacc
      121 tttcgggttt aaagcacttc aaaatggaat tgaagagaga ttgtgaaagt acatcagaca
      181 gaatcacatt ttgatagcat tcacttcact cagatgcaaa cttttcagtg actataaaaa
      241 gtcataaaat aataacatta aaaaagcaaa taaattagcg agagaatttt ttgacaaaaa
      301 gaaagaagag tgatagagaa gaagggaatg cttgaaagga ttttgcattt atctcatgga
      361 ttttcaacac ttggatcaat atgagcagtt gagttgagac aattaaatcg tacagcagcg
      421 cacaaagttg gtctcaggcc agctgtttga actaattcca tcaacggatg gatcgcacca
      481 cgagactcat aataattcag cagcaaggag aacagtgcag agttcaatga taagagcata
      541 ttctggcatg ggtcaatgta aaggacatgg gcgaaaatct gtgcaagaaa tatcaagcca
      601 atcaaaccct tgcaaacaag aagcaggata gtttttgacg agacagaaaa tcttccttgc
      661 ttaatttcaa atgggctaaa aaaaattaac cacaatgaag caattgatcc aataagagca
      721 ataacatgcg caaaccgatt tacccataca ttttcaggca gatcaagata catgtagatt
      781 ttgaaaatct ttacaagagc aagtaaaaat ccgactatga tgttccacaa aaatatgaaa
      841 atcatcgaaa attttaagtt ttggaaatca aagcgcatat tttttctac
//
