LOCUS       sequence-80339-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniMos transposable element with HygroR, R4-R3 Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1655
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(144..267)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(275..292)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(275..291)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
      181 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      241 ctcgttcaac tttattatac atagttgata attcactggc cgtcgtttta caggccggat
      301 atgaagatga acgcaaggag cctgatgcag cgactcttgc taggagatca tcagaaagct
      361 tatatttgat tagtgacgtc gcatcaaagc tgaacacata aattctttaa tattttatat
      421 ccccatatta tgcacctgag aggaatgaag aaatttgctg atactcccgg tgaattcatg
      481 tcttgatggt tgtgcgcatt gtggtaatat ctgaaaaatt tcgaagtcag ttttctgata
      541 aggcaacaac taataaattg agaatcaatt tacccttaag gaacgatgtt cgcttctgaa
      601 tttattaata aatttttccg taattcaatt caaaattgtc atatttaaga aaaaaacatc
      661 aaaaatgaca aattcaatat tttaattaat ttttgctttc gtcgtaaatc tacacacgcg
      721 tctcttccgt gcgagagtcc aagccagcag ccaaattcgt tgactgagta ttcaacgttt
      781 atacgttgtc ggcaacgaga aataggaaaa tgcatcggga aatgttcttt tttcga
//
