LOCUS       sequence-80343-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-cerFP from internal CAG 
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.CAG.H2B-cerFP.W.SINloxP
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(33..746,747..749,750..752))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     427..446
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag tattacttgt acagctcgtc catgccgaga
       61 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      121 tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      181 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      241 ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      301 acgttgtcgc tgatggcgtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      361 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      421 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      481 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      541 cactgcacgc cccaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      601 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      661 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      721 ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc cttagcgctg
      781 gtgtacttgg tgatggcctt agtaccctcg gacacggcgt gcttggccaa ctccccaggc
      841 agcagcaggc gcacggccgt ctggatctcc ctggaggtga tggtcgagcg cttgttgtaa
      901 tgcgccaggc gggaagcctc acctgcgatg cgctc
//
