LOCUS       sequence-80344-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniMos transposable element with HygroR, R4-R3 Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1655
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(116..239)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(259..275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(259..275)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(764..783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggagagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
       61 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
      121 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
      181 taatatattg atatttatat cattttacgt ttctcgttca acttttctat acaaagttga
      241 tagcttggcg taatcatggt catagctgtt tcctggatcc ggccgcagct gttttcttca
      301 aatgaaaaca gaaaattaat actgtccgcg tttgctcttt attcggcacg aaactcgaca
      361 tgttgactgc actgagagta aacaattatg acgctcaatt cgcgccaaac tatggtggtt
      421 cgacagtcaa ggttgacact tcacaaggtc aaagttttat gacaatcgat aaatatttac
      481 gtttgcgaga catctatatg ttcgaaacga catttcatac ttgtacacct gatagaattc
      541 ctcgagacgt acggtgcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaagac caggaaccgt
      661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
//
