LOCUS       sequence-80351-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based, Golgi-targeted kinase activity reporter for PKD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kunkel MT, Newton AC
  TITLE     Calcium transduces plasma membrane receptor signals to produce 
            diacylglycerol at Golgi membranes.
  JOURNAL   J Biol Chem. 2010 Jul 23;285(30):22748-52. doi: 
            10.1074/jbc.C110.123133. Epub 2010 Jun 2.
  PUBMED    20519514
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.C110.123133"; journalName: "J Biol Chem"; date: 
            "2010-07-23- 23"; volume: "285"; issue: "30"; pages: "22748-52"
COMMENT     Sequence Label: Golgi-DKAR
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(30..743,744..746))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     424..443
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggtgacacta tagaataggg ccctctagat tacttgtaca gctcgtccat gccgtgagtg
       61 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      121 ttgctcagct tggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      181 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      241 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      301 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      361 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      421 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
      481 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac
      541 atcaggccgt agccgaaggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      601 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      661 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      721 gtgaacagct cctcgccctt gctcactcca ccagatccac cttcggagac agctgcagtc
      781 agttgcctcg acagacctcc ggatcctcct aactttttca ccaaatcttt ttcttcggct
      841 gtttgcttaa gtactactct ttgttcttgt aacataccta atccctcgtt aaacagacct
      901 gtagtatcgt taatttccac tttaaagcc
//
