LOCUS       sequence-80355-e         436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Kawaoka S, Hayashi N, Suzuki Y, Abe H, Sugano S, Tomari Y, Shimada 
            T, Katsuma S
  TITLE     The Bombyx ovary-derived cell line endogenously expresses 
            PIWI/PIWI-interacting RNA complexes.
  JOURNAL   RNA. 2009 Jul;15(7):1258-64. doi: 10.1261/rna.1452209. Epub 2009 May
            21.
  PUBMED    19460866
REFERENCE   2  (bases 1 to 436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1452209"; journalName: "RNA"; date: "2009-07"; volume: 
            "15"; issue: "7"; pages: "1258-64"
COMMENT     Sequence Label: pIZ-Flag6His-Siwi
FEATURES             Location/Qualifiers
     source          1..436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    181..310
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     CDS             complement(328..345)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(355..396)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gcatcgatcc
      181 cacgcgcttg aaaggagtgt gtaaatggac aatgttgtta caataaaagt tgaaattatt
      241 ttataaaatt tattaaaaac atatttaaac ataacatatt gtattaaaac atgacaatac
      301 aaactaagat ttagtcagat aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga
      361 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact
      421 cgagcggccg cggaac
//
