LOCUS       sequence-80360-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate array vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: pFUS2_a1a
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    120..141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(156..161,162..179,180..186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    194..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     199..221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     218..234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     218..234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             230..712
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLGVR
                     LMRRLRLAVVLQRRDWENIHASVNARVGNCQASNKTKGSVGRLGLSFYLLFVGERSP"
     primer_bind     253..273
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        255..273
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(320..339)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(321..339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(349..365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(559..575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      639..710
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc
       61 tagacgtctc gggtcaggtg agaccaccgg tggaaagcgg gcagtgagcg caacgcaatt
      121 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      181 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      241 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagga cctcgagggg
      301 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
      361 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      421 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      481 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgccggtc tcggggtcag
      541 gttgatgaga cgcttaagac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca
      601 tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      661 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctga
//
