LOCUS       sequence-80371-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DICE donor plasmid with the FOXA2 and OTX2 transcription factors. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attPC-FO
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..76)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 tgctcaccat accggtggga cctgggttgc tctcaacatc tccagccaat ttcaagagag
       61 cataattagt acactgtacg tacaaaacct ggaatttcca cgaggatgtc tgatctttat
      121 aatccaagca gtcagcattg aagttaagct tccaggaggc agtttggtcc ttataatcca
      181 agcaatcagt ggttgagtta aaacccaagc ttgaagctcc atatccctgg gtggaaagag
      241 aagctgggga ctgattgaga tggctggtga ctgcattggt acccatggga ctgagtgtgg
      301 cccctggtcc gggaagctgg tgatgcatag gggtcaaata tgatccacag tccatgcccc
      361 caaagtagga agttgagcca gcatatcctt gactataacc tgaagcctga gtataggtca
      421 tgggatagga cctctgcatg caggaagagg aggtggacaa gggatctgac agtggggaga
      481 tggaagctgg gctccagata gacacaggag cactgctgct ggcaatggtc gggactgagg
      541 tgctagaggg gggagtgaat tggccacttg ttccactctc tgaactcact tcccgagctg
      601 gagatgtctt ctttttggca ggtctcactt tgttttgacc tccattctgc tgttgttgct
      661 gttgttggcg gcacttagct cttcgattct taaaccatac ctgcaccctc gactcgggca
      721 agttgatttt cagtgccacc tcctctcgca tgaagatgtc tgggtaccgg gtcttggcaa
      781 acagtgcttc cagcacatct agctgcgccc gagtgaacgt cgtcctctcc cggcgctgtt
      841 tccgg
//
