LOCUS       sequence-80372-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Bxb1 integrase in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: pCMV-Bx
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        65..83
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     65..82
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcctaggc ttttgcaaaa
       61 agctatttag gtgacactat agaaggtacg cctgcaggta ccggtccgga attcgccctt
      121 atgagagccc tggtagtcat ccgcctgtcc cgcgtcaccg atgctacgac ttcaccggag
      181 cgtcagctgg agtcttgcca gcagctctgc gcccagcgcg gctgggacgt cgtcggggta
      241 gcggaggatc tggacgtctc cggggcggtc gatccgttcg accggaagcg cagaccgaac
      301 ctggcccggt ggctagcgtt cgaggagcaa ccgttcgacg tgatcgtggc gtaccgggta
      361 gaccggttga cccgatcgat ccggcatctg cagcagctgg tccactgggc cgaggaccac
      421 aagaagctgg tcgtctccgc gaccgaagcg cacttcgata cgacgacgcc gtttgcggcg
      481 gtcgtcatcg cgcttatggg aacggtggcg cagatggaat tagaagcgat caaagagcgg
      541 aaccgttcgg ctgcgcattt caatatccgc gccgggaaat accgaggatc cctgccgccg
      601 tggggatacc tgcctacgcg cgtggacggg gagtggcggc tggtgccgga ccctgtgcag
      661 cgagagcgca tcctcgaggt gtatcaccgc gtcgtcgaca accacgagcc gctgcacctg
      721 gtggcccacg acctgaaccg gcgtggtgtc ctgtcgccga aggactactt cgcgcagctg
      781 caaggccgcg agccgcaggg ccgggagtgg tcggctaccg cgctgaagcg atcgatgatc
      841 tccgaggcga tgctcgggta cgcgactctg aacggtaaga ccgtccgaga cgacgacgga
      901 gccc
//
