LOCUS       sequence-80373-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate array vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: pFUS2_b1
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    118..139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(154..159,160..177,178..184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     197..219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     216..232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     216..232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             228..539
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLGV"
     primer_bind     251..271
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        253..271
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(318..337)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(319..337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(347..363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(550..566)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      630..716
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc
       61 tagacgtctc ggggtctgag accaccggtg gaaagcgggc agtgagcgca acgcaattaa
      121 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      181 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      241 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggaggacc tcgagggggg
      301 gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
      361 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      421 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      481 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgccggtctc ggggtctgag
      541 acgcttaaga ctggccgtcg ttttacaacg tcgtgactgg gaaaacatcc atgctagcgt
      601 taacgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcggaagact
      661 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      721 cgggagcgga tttgaacgtt gtgaagcaac g
//
