LOCUS       sequence-80374-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian phiC31 integrase expression vector. For use in 
            applications where pseudosite integration is not desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: pCS-kI
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        69..87
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     69..86
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgacctccat agaagacacc gggaccgatc cagcctccgg actctagcct aggcttttgc
       61 aaaaagctat ttaggtgaca ctatagaagg tacgcctgca ggtaccggtc cggaattccc
      121 gggtcgacga gctcactagt cgtagggtcg ccaccatgga cacgtacgcg ggtgcttacg
      181 accgtcagtc gcgcgagcgc gaaaattcga gcgcagcaag cccagcgaca cagcgtagcg
      241 ccaacgaaga caaggcggcc gaccttcagc gcgaagtcga gcgcgacggg ggccggttca
      301 ggttcgtcgg gcatttcagc gaagcgccgg gcacgtcggc gttcgggacg gcggagcgcc
      361 cggagttcga acgcatcctg aacgaatgcc gcgccgggcg gctcaacatg atcattgtct
      421 atgacgtgtc gcgcttctcg cgcctgaagg tcatggacgc gattccgatt gtctcggaat
      481 tgctcgccct gggcgtgacg attgtttcca ctcaggaagg cgtcttccgg cagggaaacg
      541 tcatggacct gattcacctg attatgcggc tcgacgcgtc gcacaaagaa tcttcgctga
      601 agtcggcgaa gattctcgac acgaagaacc ttcagcgcga attgggcggg tacgtcggcg
      661 ggaaggcgcc ttacggcttc gagcttgttt cggagacgaa ggagatcacg cgcaacggcc
      721 gaatggtcaa tgtcgtcatc aacaagcttg cgcactcgac cactcccctt accggaccct
      781 tcgagttcga gcccgacgta atccggtggt ggtggcgtga gatcaagacg cacaaacacc
      841 ttcccttcaa gccgggcagt caagccgcca ttcacccggg cagcatcacg g
//
