LOCUS       sequence-80375-e         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate array vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: pFUS2_b2
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    122..143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(158..163,164..181,182..188)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    196..212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     201..223
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     220..236
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     220..236
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             232..543
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLGV"
     primer_bind     255..275
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        257..275
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(322..341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(323..341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(351..367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(554..570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagacgt ctcggggtca tgagaccacc ggtggaaagc gggcagtgag cgcaacgcaa
      121 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      181 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      241 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg
      301 gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
      361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      481 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgccgg tctcggggtc
      541 tgagacgctt aagactggcc gtcgttttac aacgtcgtga ctgggaaaac atccatgcta
      601 gcgttaacgc gagagtaggg aactgccagg catca
//
