LOCUS       sequence-80376-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the H11-L2 TALEN in mammalian cells. This TALEN was 
            coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: MR015-H11-L2-TALEN
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..71
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             78..98
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
       61 acgatgacaa gatggccccc aagaagaaga ggaaggtggg cattcaccgc ggggtaccta
      121 tggtggactt gaggacactc ggttattcgc aacagcaaca ggagaaaatc aagcctaagg
      181 tcaggagcac cgtcgcgcaa caccacgagg cgcttgtggg gcatggcttc actcatgcgc
      241 atattgtcgc gctttcacag caccctgcgg cgcttgggac ggtggctgtc aaataccaag
      301 atatgattgc ggccctgccc gaagccacgc acgaggcaat tgtaggggtc ggtaaacagt
      361 ggtcgggagc gcgagcactt gaggcgctgc tgactgtggc gggtgagctt agggggcctc
      421 cgctccagct cgacaccggg cagctgctga agatcgcgaa gagaggggga gtaacagcgg
      481 tagaggcagt gcacgcctgg cgcaatgcgc tcaccggtgc ccccctgaac ctgaccccgg
      541 accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc
      601 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
      661 ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
      721 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
      781 agcaagcgct cgaaacggtg cagcggctgt t
//
