LOCUS       sequence-80379-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 
            locus, this TALEN was coexpressed with H11-L2 (plasmid #51554)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: MR015-H11-R2-TALEN
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..88
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caagctggct agcgccacca tggactacaa agaccatgac ggtgattata aagatcatga
       61 catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga aggtgggcat
      121 tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac agcaacagga
      181 gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc ttgtggggca
      241 tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc ttgggacggt
      301 ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg aggcaattgt
      361 aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga ctgtggcggg
      421 tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga tcgcgaagag
      481 agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca ccggtgcccc
      541 cctgaacctg accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc
      601 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgactccgga
      661 ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg
      721 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
      781 cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cg
//
