LOCUS       sequence-80380-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  p2attNG landing pad plasmid with 400 bp homology arms to the human 
            H11 locus (for use with TALEN-assisted recombination)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attNG-H11-short
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1..22)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(262..281)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     609..630
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
       61 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      121 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      181 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      241 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      301 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      361 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      421 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
      481 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      541 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
      601 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      661 gagctttaca agtaagaatt ctagataact gatcataatc agccatacca catttgtaga
      721 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      781 tgcaattgtt gttgttaact tgtttattgc a
//
