LOCUS       sequence-80381-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai
            H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A,
            Hayashi S, Yamamoto T
  TITLE     Efficient TALEN construction and evaluation methods for human cell 
            and animal applications.
  JOURNAL   Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037.
  PUBMED    23388034
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2013 Feb 6. doi: 10.1111/gtc.12037."
COMMENT     Sequence Label: pCAGGS-TAL-NC2
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..125
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             135..158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             159..185
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
       61 tcatcatttt ggcaaagaat tccgccacca tggcttcctc ccctccaaag aaaaagagaa
      121 aggttgcggc cgctgactac aaggatgacg acgataaaag ttggaaggac gcaagtggtt
      181 ggtctagaat gcatgcggcc ccgcgacggc gtgctgcgca accctccgac gcttcgccgg
      241 ccgcgcaggt ggatctacgc acgctcggct acagtcagca gcagcaagag aagatcaaac
      301 cgaaggtgcg ttcgacagtg gcgcagcacc acgaggcact ggtgggccat gggtttacac
      361 acgcgcacat cgttgcgctc agccaacacc cggcagcgtt agggaccgtc gctgtcacgt
      421 atcagcacat aatcacggcg ttgccagagg cgacacacga agacatcgtt ggcgtcggca
      481 aacagtggtc cggcgcacgc gccctggagg ccttgctcac ggatgcgggg gagttgagag
      541 gtccgccgtt acagttggac acaggccaac ttgtgaagat tgcaaaacgt ggcggcgtga
      601 ccgcaatgga ggcagtgcat gcatcgcgca atgcactgac gggtgccccc ctggagacgg
      661 gcgccgctac agggcgcgtc ccattcgcca ttc
//
