LOCUS       sequence-80386-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai
            H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A,
            Hayashi S, Yamamoto T
  TITLE     Efficient TALEN construction and evaluation methods for human cell 
            and animal applications.
  JOURNAL   Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037.
  PUBMED    23388034
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2013 Feb 6. doi: 10.1111/gtc.12037."
COMMENT     Sequence Label: pCAGGS-TAL-NC2
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(16..35)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(17..35)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(45..62)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(45..61)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(54..76)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(697..723)
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             complement(724..747)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
       61 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      181 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cccgtctcca ggggggcacc
      241 cgtcagtgca ttgcgcgatg catgcactgc ctccattgcg gtcacgccgc cacgttttgc
      301 aatcttcaca agttggcctg tgtccaactg taacggcgga cctctcaact cccccgcatc
      361 cgtgagcaag gcctccaggg cgcgtgcgcc ggaccactgt ttgccgacgc caacgatgtc
      421 ttcgtgtgtc gcctctggca acgccgtgat tatgtgctga tacgtgacag cgacggtccc
      481 taacgctgcc gggtgttggc tgagcgcaac gatgtgcgcg tgtgtaaacc catggcccac
      541 cagtgcctcg tggtgctgcg ccactgtcga acgcaccttc ggtttgatct tctcttgctg
      601 ctgctgactg tagccgagcg tgcgtagatc cacctgcgcg gccggcgaag cgtcggaggg
      661 ttgcgcagca cgccgtcgcg gggccgcatg cattctagac caaccacttg cgtccttcca
      721 acttttatcg tcgtcatcct tgtagtcagc ggccgcaacc
//
