LOCUS       sequence-80393-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DICE donor plasmid without neural transcription factors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attPC
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    51..120
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    324..548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     537..554
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(851..870)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcgaattggg taccgggccc cccctcgagg tcgacgatgt aggtcacggt ctcgaagccg
       61 cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc
      121 tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc
      181 accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt
      241 gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg
      301 gcgggcatgt cgacggtatc gatccataga gcccaccgca tccccagcat gcctgctatt
      361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
      421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
      481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
      541 aggcacagtc gagggctcga gtcgcgcgcg atttacttgt acagctcgtc catgccgccg
      601 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
      661 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
      721 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
      781 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
      841 cagcccatgg tcttcttctg cattacgggg cc
//
