LOCUS       sequence-80397-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for expression of human TLR4 299/399 SNP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Guo J, Loke J, Zheng F, Hong F, Yea S, Fukata M, Tarocchi M, Abar 
            OT, Huang H, Sninsky JJ, Friedman SL
  TITLE     Functional linkage of cirrhosis-predictive single nucleotide 
            polymorphisms of Toll-like receptor 4 to hepatic stellate cell 
            responses.
  JOURNAL   Hepatology. 2009 Mar;49(3):960-8. doi: 10.1002/hep.22697.
  PUBMED    19085953
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/hep"; 
            journalName: "Hepatology"; date: "2009-03"; volume: "49"; issue: 
            "3"; pages: "960-8"
COMMENT     Sequence Label: hu-TLR4 299/399 dual snp flag pDEST40
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(43..60)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(70..111)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(179..202)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagaaggc acagtcgagg ctgatcagcg ggtttaaact caatggtgat ggtgatgatg
       61 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
      121 cgggccctct agatcaacca ctttgtacaa gaaagctggg tcgaattcgc ccttgttact
      181 tatcatcgtc gtccttgtag tcgatagatg ttgcttcctg ccaattgcat cctgtaccca
      241 ctgttccttc tggattccat gatttaccat ccagcagggc ttttctgagt cgtctccaga
      301 agatgtgccg ccccaggaca ctgtcctccc actccaggta agtgttcctg ctgagaaggc
      361 ggtacagctc cacctgctgc ctgagcaggg tcttctccac cttctgcagg acaatgaaga
      421 tgataccagc acgactgctc agaaactgcc aggtctgagc aatctcatat tcaaagatac
      481 accagcggct ctggatgaag tgctgggaca ccacaacaat cacctttcgg cttttatgga
      541 aaccttcatg gatgatgttg gcagcaatgg ccacaccggg aataaagtct ctgtagtgaa
      601 ggcagagctg aaatggaggc accccttctt ctaaattctt tactagctca ttccttaccc
      661 agtcctcatc ctggcttgag tagataacaa aggcatcata gatgttttca cctctaccat
      721 actttatgca gccagcaaga agcatcaggt gaaaatagaa cttatagacc agaactgcta
      781 caacagatac tacaagcaca ctgaggaccg acacaccaat gatggtctta ttcatctgac
      841 aggtgatatt caaactcagc acaggcatgc cctgcttatc tgaaggtgtt tgcacattcc
      901 attcgttcaa cttccaccaa gagctgcctc tggtccttga tccattgcag gaa
//
