LOCUS       sequence-80408-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CylB (acyl carrier protein) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Nakamura H, Hamer HA, Sirasani G, Balskus EP
  TITLE     Cylindrocyclophane biosynthesis involves functionalization of an 
            unactivated carbon center.
  JOURNAL   J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p.
            Epub 2012 Nov 2.
  PUBMED    23106426
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja308318p";
            journalName: "J Am Chem Soc"; date: "2012-11-14- 14"; volume: "134";
            issue: "45"; pages: "18518-21"
COMMENT     Sequence Label: pET-29b-CylB
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             367..384
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(437..455)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      451..498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(622..641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg aaatcgtaaa tagtcagatt
       61 aattcaatct cggtcgatgc agaaaacaat cacgcccgtg ccaagaaact acccacaaag
      121 gtagaaattc aaacctggct agttaactac ctagtggaac agctagactt aaatccagcc
      181 agagtagatg tctcaattcc ttttgaacgc tacggcattg attctgctgc tgcaatagtg
      241 ttggtagggg atttaaaaga ttggttggaa atagacctag agccaacttt aatctacgat
      301 tatccatcta ttgaagagtt agcccggcac ttgaatgatt caattaaagc agaatattca
      361 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      481 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      781 cctgatagac ggtttt
//
