LOCUS       sequence-80411-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CylI (type III PKS) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Nakamura H, Hamer HA, Sirasani G, Balskus EP
  TITLE     Cylindrocyclophane biosynthesis involves functionalization of an 
            unactivated carbon center.
  JOURNAL   J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p.
            Epub 2012 Nov 2.
  PUBMED    23106426
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja308318p";
            journalName: "J Am Chem Soc"; date: "2012-11-14- 14"; volume: "134";
            issue: "45"; pages: "18518-21"
COMMENT     Sequence Label: pET-28a-CylI
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgac ttatatagtt tctacatcaa
      121 cagcatttcc agaaaattac taccctcaaa cagttttagc tgctgcttta cgcaggtttt
      181 ttactgtcat ggagttagat ttcgatctag aacagattga tcgttttttc acaaacgtca
      241 aaatcgacgg tcgttacttc gctctaccac tagattcctt actcgatccg cccacatggg
      301 gagtaagtat cagtagaggt ttggaaaatt ctttgaactt ggctgaaacc gctatcacca
      361 aattgctgga aaaaactaat gtccaacccc aagatatatc tctgcttgcg tctgtttcta
      421 tgactccagc aattccctct ttagatgggc ggttgatgaa tcggattcca ttttcttcca
      481 ccctgaagag attgcccatg aatggagtag gttgtatggg tggagctttt ggcatttccc
      541 gcgttgctga ttaccttaaa gcacatccaa aggaagcagc aattttattt gcagtggaaa
      601 tttcttccgc tctatggcaa ggttctttgc aagcaaattt aacttcattg attcgtcgcc
      661 ttccagaaaa tccctcttta tatagtgaga ttattatgga tattatcact gctgctttgt
      721 ttgcagatgg ttgcggcgca gtgttaatgg taggaaaaga gcatccttta gctaaatcgg
      781 gactacctca agtcattgat aatcgttcct ttttagttcc taacacagta gaactaatgg
      841 ggctggacgt agttgataat ggttttagaa atattctca
//
