LOCUS       sequence-80415-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NRPS module of ClbB containing CAT domains with N-terminal 6His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Brotherton CA, Balskus EP
  TITLE     A prodrug resistance mechanism is involved in colibactin 
            biosynthesis and cytotoxicity.
  JOURNAL   J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. 
            Epub 2013 Feb 20.
  PUBMED    23406518
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja312154m";
            journalName: "J Am Chem Soc"; date: "2013-03-6- 6"; volume: "135"; 
            issue: "9"; pages: "3359-62"
COMMENT     Sequence Label: pet28a-ClbB-NRPS-NHis
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             91..123
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg acagcaaatg
      121 ggtcgcggat ccgaattcga gctccgtcga caagcttgcg gccgcatgga taatacctct
      181 ggagattttc catgtaataa gatggacacg cgtaagcagt taccgctaac accaagtcaa
      241 caggggtttt tattccattc cttaaaggat aagaaaagga gtaactacca tgagcatttt
      301 acatgcattt tttctcagca tgtagatagc gcccacttca agtgggcgct ggaaacgtta
      361 tttcgaaagc atgagtgttt tcgcactgat tataactggg agattgatga gcgcccttgt
      421 caggtggtga agaccgatgt gttgccggat atatatgtgt tagactgtga gcaagaggaa
      481 atacgttttc tactagctaa tgatgacatt atcattcctg tcccgcagga tgacggtatt
      541 gatgctataa ttcctcaact gctacaggct gatttaaaat acccattttc cttgaaaacg
      601 atcccagtcc gggcctacct tattcagtca acgaaagaaa gtgcttttat actatcatac
      661 catcatattg tgatggatgg ctggagctta tcccttttca ttaaacagtt gctccaactc
      721 tatggagcgg ctgtggtcag tggtgtgagg gatgatagcg ccattatccc ctcatctctg
      781 aaaccccttg tagacacact gtcggcccga cgtcacacct ttcagcacga ctattgggct
      841 gcatatcttc gggagggaac accaacttgt atcgtgccgc tgtcacaata tcacacagat
      901 actgaagccg a
//
